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2024-08-08YFP激发光波长和YFP的发射波长是多少?
编辑:激发光源事业部时间:2019-09-05 10:33:20浏览21912 次
黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm,更大发射波长为527 nm。
如何快速观察到EGFP在动植物体内的表达?
美国路阳生产一款便携式荧光蛋白激发光源,能够方便快捷观察EGFP在动植物体内的表达。大家免费提供样机供科研院所试用,满意付款。如有兴趣,可添加微信号(luyor01)联系。
EGFP在烟草上的表达
LUYOR-3415RG便携式荧光蛋白激发光源
红色荧光蛋白在斑马鱼上的表达
绿色荧光蛋白在果蝇上的表达
绿色荧光蛋白在老鼠幼崽上的表达
黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm,更大发射波长为527 nm。excitation wavelength and the emission wavelength for yfp.
Yellow Fluorescent Proteins Excitation Emission
如何能看到YFP黄色荧光蛋白的表达?
美国路阳生产有多款便携式激发光源,无需荧光显微镜就能轻松观察筛选黄色荧光蛋白表达阳性。LUYOR-3260CY便携式YFP激发光源能直接分选YFP表达阳性的植株,能够分选愈伤组织、植株、种子、菌落等。如欲进一步了解便携式荧光蛋白激发光源,可直接联系上海路阳生物技术有限企业的销售客服。
Yellow Fluorescent Proteins/常用黄色荧光蛋白的激发波长和发射波长
YFP种类 | Excitation 激发光波长(nm) | Emission发射光波长(nm) |
EYFP | 514 | 527 |
Topaz | 514 | 527 |
Venus | 515 | 528 |
mCitrine | 516 | 529 |
YPet | 517 | 530 |
TagYFP | 508 | 524 |
PhiYFP | 525 | 537 |
ZsYellow1 | 529 | 539 |
mBanana | 540 | 553 |
LUV-40A荧光蛋白观察眼镜是用于观察黄色荧光蛋白表达位置
在绿色荧光蛋白的晶体结构研究中发现203位苏氨酸接近生色团后,对黄色荧光蛋白家族的研究才开始。将203位苏氨酸突变为色氨酸可以稳定生色团在激发状态的偶极矩,这样可以使激发光和发射光的波长均增加20nm。并进一步改造成增强黄色荧光蛋白(EYFP)。EYFP是目前应用最广最亮的荧光蛋白之一。EYFP的亮度和发射光谱的有机结合使得这一荧光探针在荧光多色成像中得到很好的应用。EYFP还可以与增强青色荧光蛋白(ECFP)或GFP2一起应用到FRET中。然而,黄色荧光蛋白也存在一些问题,如对酸性条件非常敏感,pH6.5时会失去近50%的荧光。除此之外,EYFP对氯离子非常敏感,而且光漂白现象比绿色荧光蛋白还严重。
对黄色荧光蛋白的结构研究已经解决了上述问题。Citrine变种的亮度与黄色荧光蛋白相当,而且可以抗光漂白、酸性条件和其他环境因素的改变。一种珊瑚蛋白,ZsYellow1,产生的真正的黄光是多色荧光实验中的理想选择。像ZsGreen1一样,这个衍生物也不能像EYFP产生融合蛋白,还易于产生四聚物。越来越多黄色荧光蛋白的衍生物已经用于FRET研究中,在其他研究中也表现出巨大的潜力。
Yellow Fluorescent Proteins
Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.
To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.
YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.
黄色荧光蛋白的激发波长和发射波长
Fluorochrome | Excitation Wavelength (Nanometers) | Emission Wavelength (Nanometers) |
---|---|---|
Acridine Orange (Bound to DNA) | 500 | 526 |
Alexa Fluor 488 | 495 | 519 |
Alexa Fluor 500 | 503 | 525 |
Aminofluorescein | 497 | 519 |
Astrazon Orange R | 470 | 540 |
Aurophosphine | 450-490 | 515 |
BCECF (Biscarboxyethylcarboxyfluorescein) | 505 | 530 |
BODIPY 492/515 (Difluoroboradiazaindacene) | 490 | 515 |
BODIPY 493/503 | 500 | 506 |
BODIPY 500/510 | 509 | 515 |
BODIPY 505/515 | 502 | 510 |
BODIPY FL | 503 | 512 |
Calcein | 494 | 517 |
Calcium Green | 506 | 533 |
CellTracker Green CMFDA (Chloromethylfluorescein Diacetate) | 492 | 517 |
CFDA (Carboxyfluorescein Diacetate) | 495 | 520 |
Chinacrine | 450-495 | 515 |
Cl-NERF | 514 (High pH) | 540 (High pH) |
CyQuant GR | 480 | 520 |
DCFH (Carboxydichlorofluorescein) | 505 | 535 |
DiBAC4(3) (Bisdibutylbarbituric Acid) | 493 | 516 |
DiO (Dipentyloxacarbocyanine Iodide) | 484 | 502 |
DiOC16(3) | 484 | 501 |
DiOC18(3) | 484 | 501 |
DiOC2(3) | 482 | 497 |
DiOC5(3) | 484 | 500 |
DiOC6(3) | 484 | 511 |
DiOC7(3) | 482 | 504 |
DM-NERF | 510 (High pH) 497 (Low pH) | 536 (High pH) 527 (Low pH) |
DTAF (Fluorescein Dichlorotriazine) | 494 | 520 |
FAM (Carboxyfluorescein) | 492 | 518 |
FDA (Fluorescein Diacetate) | 495 | 520 |
FITC (Fluorescein Isothiocyanate) | 494 | 518 |
FlCRhR (Cyclic AMP Fluorosensor) | 500 | 517 |
Fluo-3 | 480-506 | 520 |
Fluo-4 | 494 | 516 |
Fluo-4FF | 494 | 518 |
Fluo-5F | 494 | 516 |
Fluo-5N | 493 | 518 |
Fluorescein | 494 | 518 |
Fluorescein Sulfonic Acid | 476 | 519 |
FluoroEmerald | 495 | 524 |
FluorX | 494 | 520 |
FluoZin-1 | 495 | 517 |
FluoZin-2 | 494 | 521 |
FluoZin-3 | 494 | 516 |
GFP-RS (Red-Shifted) | 498 | 516 |
LysoTracker Green DND-26 | 504 | 511 |
Magnesium Green | 506 | 531 |
MitoFluor Green | 490 | 516 |
MitoTracker Green FM | 490 | 516 |
NeuroTrace 500/525 | 500 | 525 |
NeuroTrace 515/535 | 515 | 535 |
Newport Green DCF | 506 | 535 |
Newport Green PDX | 490 | 518 |
Nonyl Acridine Orange | 495 | 519 |
Oligreen | 498 | 518 |
Oregon Green 488 | 496 | 524 |
Oregon Green 500 | 503 | 522 |
Oregon Green 514 | 511 | 530 |
PFB-F (Pentafluorobenzoyl Fluorescein) | 492 | 516 |
Phen Green FL | 492 | 517 |
Phen Green SK | 507 | 532 |
Pico Green | 502 | 523 |
PKH67 | 496 | 520 |
Rhodamine 110 | 496 | 520 |
Rhodamine 123 | 507 | 529 |
Rhodamine Green | 502 | 527 |
Sodium Green | 506 | 532 |
SpectrumGreen 1 | 497 | 524 |
SpectrumGreen 2 | 509 | 538 |
Stachyose Fluorescein | 491 | 516 |
SYBR Gold | 495 | 537 |
SYBR Green I | 498 | 522 |
SYBR Green II | 492 | 513 |
SYTO 11 | 508 | 527 |
SYTO 12 | 499 | 522 |
SYTO 13 | 488 | 509 |
SYTO 16 | 488 | 518 |
SYTO 21 | 494 | 517 |
SYTO 23 | 499 | 520 |
SYTO 24 | 490 | 515 |
SYTOX Green | 504 | 523 |
TAMRA (Carboxytetramethylrhodamine) | 504 | 529 |
YFP/EYFP (Enhanced Yellow Fluorescent Protein) | 512 | 529 |
YOYO-1, YO-PRO-1 | 491 | 509 |
黄光波长 :
黄光中心波长:570纳米;黄光波长范围:577nm-597nm,
更多黄光波长和可见光波长先容可浏览:《可见光光谱》